Welcome!
Our mission is to provide researchers with a single facility where they can access a comprehensive range of resources and services for gene transfer purposes. The Core constructs purifies, and titers custom recombinant adenovirus, adeno-associated virus, and lentivirus. The core is administered via the Department of Physiology and Biophysics and is physically located in COMRB2082. For inquiries about specific issues and to initiate a service request, please contact Dr. Seock-Won Youn at swyoun93@uic.edu, or 312-355-3682 in COMRB room 2160.
Adenovirus:
Track 3: Amplification of adenovirus from customer's virus, 2 ~ 4 weeks, volume: 1-2 ml, Titer: >1X10^10 pfu/ml.
Track 4: Amplification of adenovirus from customer's virus, 4 ~ 8 weeks, volume: 1-2 ml, Titer: >1X10^11 pfu/ml.
Generation of adenovirus – individual investigators construct the shuttle vector, verify, and supply the DNA to the core. Core does recombination and virus generation.
Adeno-Associated Virus (AAV): The core has AAV serotypes (1, 2, 8, and 9) currently.
Track 2: Investigator provides sufficient verified AAV shuttle DNA. 1-2 months, ~0.5ml, Titer: >1E13 vector genomes (Vg/ml)
Track 3: Investigator provides sufficient verified AAV shuttle DNA. 1-2 months, ~0.5ml, Titer: >1E13 vector genomes (Vg/ml)
Lenti-/Retro-virus:
Virus titration
The viral vector core can measure your virus titer ( 1-2 weeks).
Subcloning:
The Core supports services of subclone for virus vectors with knockdown, knockout, overexpression, tagging, mutation, etc.
Users are strongly encouraged to contact Dr. Youn in the planning process of a project.
Premade viruses:
The Core can amplify some control viruses such as Adv-CMV-GFP, Lenti-PGK-eGFP, Lenti-PGK-RFP, and AAV-CMV-GFP. Please contact Dr. Youn to get more information.
Please note, Investigators from Northwestern University and the University of Chicago are provided internal rates as all UIC users.
All other users can access services but, at either external academic or external non-academic rate.
Director:
Seock-Won Youn, 312-355-3682, swyoun93@uic.edu
Tech:
Braulio Aguilar Lugo, baguil20@uic.edu
Monday – Friday
9:00 am-5:00 pm
College of Medicine, COMRB 2160
The University of Illinois Chicago
909 South Wolcott Ave. Chicago, IL 60612
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Seock-Won Youn |
Director
|
312-355-3682
|
swyoun93@uic.edu
|
Chicago
|
| Cesar Alvarado |
Admin
|
312-996-3836
|
calvar3@uic.edu
|
Chicago
|
| Braulio Aguilar Lugo |
Tech
|
312-355-3686
|
baguil20@uic.edu
|
Chicago
|
| Service list |
| ► Consultant (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Additionl consulation fee |
We can discuss your projects for viruses. $50.00 per hour rate. |
Inquire | |
| ► Cloning service (3) | |||
| Name | Description | Price | |
| Cloning for gene expression |
Core does subclone for virus vectors with overexpression, tagging, etc. Dependent on the complexes, we will charge an additional fee.
|
Inquire | |
| Cloning for gene knockdown or knockout |
Core does cloning for virus vectors with knockdown (shRNA) and knockout (gRNA). |
Inquire | |
| Cloning for Genetic mutation of interesting genes |
Core does point mutation for interesing gene in virus vectors. If you want several different regional mutation, please discuss it with Dr. Youn. |
Inquire | |
| ► Adenovirus (4) | |||
| Name | Description | Price | |
| Adenovirus track 1 |
Crude amplification of adenovirus from customer's virus, ~1 week time, volume: 0.5~1ml, No titration. |
Inquire | |
| Adenovirus track 2 |
Amplification of adenovirus from customer's virus, 2 ~ 4 weeks time, volume: 1-2 ml, Titer: >1X10^9 pfu/ml. |
Inquire | |
| Adenovirus track 3 |
Amplification of adenovirus from customer's virus, 2 ~ 4 weeks time, volume: 1-2 ml, Titer: >1X10^10 pfu/ml. |
Inquire | |
| Homologue recombination and adenovirus synthesis |
The customer provides a shuttle vector around 1 ug and their plasmid map. The Core does the homolog recombination from the customer's shuttle vector and virus generation (2~3 weeks). To amplify an adenovirus, the customers need to request other amplification services (Track 1 to 4), depending on their purpose. The plasmid amplification and the cloning of the shuttle vector are separated from this service. |
Inquire | |
| ► Lenti-/Retrovirus (2) | |||
| Name | Description | Price | |
| Lenti-/Retro-virus track 1 |
Crude virus amplification from customer's vector, ~ 2 week time, volume: ~10ml of lentivirus media or ~100ul of 100x concentration with addtional charge ($20), No titration. The customer provides ~20ug of expression vector per virus. If the customer don't have enuough expression vector, the core will amplify the plasmid and it will be added to the service charges ($30, transformation and mini-prep). |
Inquire | |
| Lenti-/Retro-virus track 2 |
Amplification of Lenti-/Retro-virus from customer's vector with concentration, ~ 2 week time, volume: 0.1 ~ 1ml, Titer: >1X10^8 TU/ml. If the customer doesn't provide enough expression vector (300ug), the core will amplify the plasmid and it will be added to the service charges ($70, transformation and midi-prep). The current concentration service is a PEG-based procedure. If the customer wants to use ultracentrifuge-based concentration without PEG precipitation, we will add the additional Ultracentrifuge service charge (Internal:$60 and exteranl:$70). |
Inquire | |
| ► AAV (2) | |||
| Name | Description | Price | |
| AAV (Adeno-Associated Virus) track 2 |
The investigator provides sufficient verified AAV shuttle DNA. 1-2 months time, 0.5 ~ 0.7 ml, Titer: >1E12 vector genomes (Vg/ml). It will be added to the AAV shuttle vector amplification price ($70 per a plasmid, transformation and midiprep), if the customer don't have enough plasmid DNA (200ug). |
Inquire | |
| AAV (Adeno-Associated Virus) track 3 |
The investigator provides sufficient verified AAV shuttle DNA. 1-2 months time, 0.5 ~ 0.7 ml, Titer: >1E13 vector genomes (Vg/ml). It will be added to the AAV shuttle vector amplification price ($70 per a plasmid, transformation and midiprep), if the customer don't have enough plasmid DNA (450ug). |
Inquire | |
| ► Virus titration (3) | |||
| Name | Description | Price | |
| AAV Titration |
The core provides the titration of the adeno-associated virus with qPCR and the value is the genomic copy (GC)/ml. |
Inquire | |
| The titration of virus (Adenovirus, lentivirus, and retrovirus) with a fluorescence report gene |
The core provides virus titration using a reporter gene such as GFP in your virus. The titer value is a plaque-forming unit (pfu)/ml for adenovirus and a transducing unit (TU)/ml for Lenti-/Retro-virus.
|
Inquire | |
| Titration of virus (Adenovirus, lentivirus, and retrovirus) without a fluorescence report |
The core provides virus titration with immunostaining for Adenovirus and qPCR for Lenti-/Retro-virus. The titer value is a plaque-forming unit (pfu)/ml for adenovirus and a transducing unit (TU)/ml for Lenti-/Retro-virus.
|
Inquire | |
| ► Premade viruses (1) | |||
| Name | Description | Price | |
| AAV-CMV-GFP |
We generated AAV-GFP with CMV promoter in AAV serotype 1. The titer is over 1X10^12 vp/ml and the volume is 50ul. If you are interesting for this, please let us know. |
Inquire | |
