Viral Vector Core

The investigator provides sufficient verified AAV shuttle DNA. 1-2 months time, 0.5 ~ 0.7 ml, Titer: >1E13 vector genomes (Vg/ml).

It will be added to the AAV shuttle vector amplification price ($70 per a plasmid, transformation and midiprep), if the customer don't have enough plasmid DNA (450ug).

The investigator provides sufficient verified AAV shuttle DNA. 1-2 months time, 0.5 ~ 0.7 ml, Titer: >1E12 vector genomes (Vg/ml).

It will be added to the AAV shuttle vector amplification price ($70 per a plasmid, transformation and midiprep), if the customer don't have enough plasmid DNA (200ug).

The core provides the titration of the adeno-associated virus with qPCR and the value is the genomic copy (GC)/ml.

Crude amplification of adenovirus from customer's virus, ~1 week time, volume: 0.5~1ml, No titration.

Amplification of adenovirus from customer's virus, 2 ~ 4 weeks time, volume: 1-2 ml, Titer: 1X10^9 ~ 1X10^10 pfu/ml.

Amplification of adenovirus from customer's virus, 2 ~ 4 weeks time, volume: 1-2 ml, Titer: 1X10^10 ~ 1X10^11 pfu/ml.

Generation of adenovirus – individual investigators construct the shuttle vector, verify, and supply the DNA to the core. 

Core does the homolog recombination and virus generation, amplification at 1 time, volume: 1-2 mls.

The plasmid amplification and the cloning of the shuttle vector is separated from this service.

Core does subclone for virus vectors with overexpression, tagging, etc.

Dependent on the complexes, we will charge an additional fee.

Core does cloning for virus vectors with knockdown (shRNA) and knockout (gRNA).

Core does point mutation for interesing gene in virus vectors.

If you want several different regional mutation, please discuss it with Dr. Youn.

We can discuss your projects for viruses.

Please send a message about what you are needed to assistant.

Thank you,

The customer provides a shuttle vector around 1 ug and their plasmid map.

The Core does the homolog recombination from the customer's shuttle vector and virus generation (2~3 weeks).

To amplify an adenovirus, the customers need to request other amplification services (Track 1 to 4), depending on their purpose.

The plasmid amplification and the cloning of the shuttle vector are separated from this service.

Crude virus amplification from customer's vector, ~ 2 week time, volume: ~10ml of lentivirus media or ~100ul of 100x concentration with addtional charge ($20), No titration.

The customer provides ~20ug of expression vector per virus.

If the customer don't have enuough expression vector, it will be added to the service charges ($30, transformation and mini-prep).

Amplification of Lenti-/Retro-virus from customer's vector with concentration, ~ 2 week time, volume: 0.1 ~ 1ml, Titer: >1X10^8 TU/ml.

If the customer doesn't provide enough expression vector (300ug), it will be added to the service charges ($70, transformation and midi-prep).

The current concentration service is a PEG-based procedure.

If the customer wants to use ultracentrifuge-based concentration without PEG precipitation, we will add the additional Ultracentrifuge service charge (Internal:$60 and exteranl:$70).

We generated AAV-GFP with CMV promoter in AAV serotype 1.

The titer is over 1X10^13 vp/ml and the volume is 50ul.

If you are interesting for this, please let us know.

The core provides virus titration using a reporter gene such as GFP in your virus.

The titer value is a plaque-forming unit (pfu)/ml for adenovirus and a transducing unit (TU)/ml for Lenti-/Retro-virus.

The core provides virus titration with immunostaining for Adenovirus and qPCR for Lenti-/Retro-virus.

The titer value is a plaque-forming unit (pfu)/ml for adenovirus and a transducing unit (TU)/ml for Lenti-/Retro-virus.

We can discuss your projects for viruses.

Please send a message about what you are needed to assistant.

Thank you,

Core does subclone for virus vectors with overexpression, tagging, etc.

Dependent on the complexes, we will charge an additional fee.

Core does cloning for virus vectors with knockdown (shRNA) and knockout (gRNA).

Core does point mutation for interesing gene in virus vectors.

If you want several different regional mutation, please discuss it with Dr. Youn.

Crude amplification of adenovirus from customer's virus, ~1 week time, volume: 0.5~1ml, No titration.

Amplification of adenovirus from customer's virus, 2 ~ 4 weeks time, volume: 1-2 ml, Titer: 1X10^9 ~ 1X10^10 pfu/ml.

Amplification of adenovirus from customer's virus, 2 ~ 4 weeks time, volume: 1-2 ml, Titer: 1X10^10 ~ 1X10^11 pfu/ml.

The customer provides a shuttle vector around 1 ug and their plasmid map.

The Core does the homolog recombination from the customer's shuttle vector and virus generation (2~3 weeks).

To amplify an adenovirus, the customers need to request other amplification services (Track 1 to 4), depending on their purpose.

The plasmid amplification and the cloning of the shuttle vector are separated from this service.

Crude virus amplification from customer's vector, ~ 2 week time, volume: ~10ml of lentivirus media or ~100ul of 100x concentration with addtional charge ($20), No titration.

The customer provides ~20ug of expression vector per virus.

If the customer don't have enuough expression vector, it will be added to the service charges ($30, transformation and mini-prep).

Amplification of Lenti-/Retro-virus from customer's vector with concentration, ~ 2 week time, volume: 0.1 ~ 1ml, Titer: >1X10^8 TU/ml.

If the customer doesn't provide enough expression vector (300ug), it will be added to the service charges ($70, transformation and midi-prep).

The current concentration service is a PEG-based procedure.

If the customer wants to use ultracentrifuge-based concentration without PEG precipitation, we will add the additional Ultracentrifuge service charge (Internal:$60 and exteranl:$70).

The investigator provides sufficient verified AAV shuttle DNA. 1-2 months time, 0.5 ~ 0.7 ml, Titer: >1E13 vector genomes (Vg/ml).

It will be added to the AAV shuttle vector amplification price ($70 per a plasmid, transformation and midiprep), if the customer don't have enough plasmid DNA (450ug).

The investigator provides sufficient verified AAV shuttle DNA. 1-2 months time, 0.5 ~ 0.7 ml, Titer: >1E12 vector genomes (Vg/ml).

It will be added to the AAV shuttle vector amplification price ($70 per a plasmid, transformation and midiprep), if the customer don't have enough plasmid DNA (200ug).

We generated AAV-GFP with CMV promoter in AAV serotype 1.

The titer is over 1X10^13 vp/ml and the volume is 50ul.

If you are interesting for this, please let us know.

The core provides the titration of the adeno-associated virus with qPCR and the value is the genomic copy (GC)/ml.

The core provides virus titration using a reporter gene such as GFP in your virus.

The titer value is a plaque-forming unit (pfu)/ml for adenovirus and a transducing unit (TU)/ml for Lenti-/Retro-virus.

The core provides virus titration with immunostaining for Adenovirus and qPCR for Lenti-/Retro-virus.

The titer value is a plaque-forming unit (pfu)/ml for adenovirus and a transducing unit (TU)/ml for Lenti-/Retro-virus.

Generation of adenovirus – individual investigators construct the shuttle vector, verify, and supply the DNA to the core. 

Core does the homolog recombination and virus generation, amplification at 1 time, volume: 1-2 mls.

The plasmid amplification and the cloning of the shuttle vector is separated from this service.