Our mission is to provide researchers with a single facility where they can access a comprehensive range of resources and services for gene transfer purposes. The Core constructs purifies, and titers custom recombinant adenovirus, adeno-associated virus, and lentivirus. The core is administered via the Department of Physiology and Biophysics and is physically located in COMRB2082. For inquiries about specific issues and to initiate a service request, please contact Dr. Seock-Won Youn at swyoun93@uic.edu, or 312-355-3682 in COMRB room 2160.
Adenovirus:
Track 3: Generation of adenovirus – individual investigators construct the shuttle vector, verify, and supply the DNA to the core. Core does recombination and virus generation, amplifies 1 time, volume: 1-2 ml, ~ 1-2 months. Titer: >1X10^9 pfu/ml.
Adeno-Associated Virus (AAV): Core has AAV serotypes (1, 2, 8, and 9) currently.
Lenti-/Retro-virus:
Virus titration
The viral vector core can measure your virus titer ( 1-2 weeks time).
Subcloning:
Core does subclone for virus vectors with knockdown, knockout, overexpression, tagging, etc.
If the size of the gene is over 2kb, we will charge $50.00 for every 1kb.
For more specific services available (promoter options, mutagenesis, fusion protein construction, etc), please contact Dr. Youn.
Users are strongly encouraged to contact Dr. Youn in the planning process of a project.
Please note, Investigators from Northwestern University and the University of Chicago are provided internal rates as all UIC users. All other users can access services but, at either external academic or external non-academic rate.
Director:
Seock-Won Youn, 312-355-3682, swyoun93@uic.edu
Tech:
Braulio Aguilar Lugo, baguil20@uic.edu
Monday – Friday
9:00 am-5:00 pm
College of Medicine, COMRB 2160
The University of Illinois Chicago
909 South Wolcott Ave. Chicago, IL 60612
Name | Role | Phone | Location | |
---|---|---|---|---|
Seock-Won Youn |
Director
|
312-355-3682
|
swyoun93@uic.edu
|
Chicago
|
Cesar Alvarado |
Admin
|
312-996-3836
|
calvar3@uic.edu
|
Chicago
|
Braulio Aguilar Lugo |
Tech
|
312-355-3686
|
baguil20@uic.edu
|
Chicago
|