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Biophysics Core



Research Resources Center (RRC) cores are back on campus to provide services and access to shared equipment (when possible), there are new guidelines issued by each core due to COVID19. Please reference each core’s new user guidelines before requesting services, projects, or scheduling equipment. All questions or inquiries must be sent by email to respective core.




Overview of Services

Understanding the function and structure of biomolecules is crucial in all areas of modern health and life sciences. Characterizing the structure and dynamics of these molecules is essential to understanding the basis of various cellular function, and the Biophysics Core at the Center for Structural Biology is to provide unimpeded access to state-of-the-art and well-maintained biophysics equipment.

The Biophysics Core offers three main categories of research and services: characterization of macromolecules using various biophysical instruments (Mass Photometry (MP), Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), and Circular Dichroism (CD)), large-scale protein production using a multi-column AKTA FPLC, and macromolecular structural biology using X-ray crystallography and Cryo-EM in collaboration.

The Biophysics Core houses a suite of various biophysical instruments and has a fully equipped wet-laboratory for molecular cloning, recombinant protein expression, and multi-step protein purification. Large-scale protein purification service is also offered to users who need purified proteins for SPR, ITC, High-Throughput Screening (HTS), and structural studies.

Hands-on training is available for long term users who want to have full access to the instruments, as well as assisted runs by a staff are offered to investigators who occasionally need to use core instruments.


   Hyun Lee, PhD ǀ Director
  • Protein over-expression & purification (25 years, 1998-present)
  • SPR (23 years, 2001-present), ITC (6 years), CD (6 years)
  • Biochemical/biophysical (protease, kinase, TSA, FP, and so on) assays (16 years, 2008-present)
  • X-ray crystallography (~10 years, on and off during 2006-present)
  • Protein NMR (8 years, 1998-1999, 2001-2007)                   

Robel Demissie, PhD ǀ Postdoctoral Research Associate

  • Protein expression & purification 
  • Mass Photometry (MP)
  • Cellular Thermal Shift Assay & Enzyme Assays and Kinetics
  • Protein-protein interaction techniques (ITC, TSA, FRET, etc)
  • Circular Dichroism (CD), Electron Paramagnetic Resonance Spectroscopy (EPSR)
  • Fluorescence Spectroscopy & UV/Fluorescence Spectroscopy

Elizabeth Mulder, PhD | Postdoctoral Research Associate

  • Protein expression & purification
  • Biochemical & Biophysical Characterization of Proteins
  • Mammalian cell culture & Live cell microscopy
  • Fluidic Force Microscopy
  • Flow cytometry & Rheology
  Yuri Song ǀ Undergraduate Research Technologist
  • Bacterial transformation in E.coli
  • Small & Large-scale protein over-expression 
  • Protein purification 
  • DNA amplification & purification

 Location and Hours

Location   Hours

900 S. Ashland Ave 

Chicago, IL  60607

  24 hours/7 Days open for the trained self-users

  Staffed: 9am - 5pm/ Mon - Fri

Links and Resources

  1. Biophysics Core Website
  2. Biophysics Core Instruments
  3. Biophysics Core FAQs
  4. Getting Started Help Site


Name Role Phone Email Location
Hyun Lee
Center for Structural Biology (CfSB)

Service list

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