Welcome!
The Research Tissue Imaging Core (RTIC) is supported by the Department of Pathology at the University of Illinois at Chicago. The RTIC's mission is to provide high-quality and high-throughput digital microscopy imaging services for reproducible, automated analysis of tissue morphological characteristics and biomarkers expression in human and animal tissue. The core offers consultations on image analysis approaches as well as training on laser capture microdissection. Pathology consultations are available upon request through the collaboration with the Department of Pathology clinical and research faculty.
General questions about sample preparation, pricing, turnaround time, and equipment scheduling should be directed to:
Imaging - Ryan Deaton (rdeaton@uic.edu)
LCM, TMA and pathology consultation - Virgilia Macias (vmacias@uic.edu)
Imaging Services:
• Brightfield and Fluorescent whole slide digital scans of fixed or frozen tissue and cytological samples
-20x, 40x and 60x magnification
-Support for Akoya Opal and Alexa Flour dyes
-Z stacking and maximum in focus projections
-Polarized light imaging
• Image analysis training and drop off service
-Deep learning segmentation and classification
-Quantification of immunohistochemical and special stains
-Spatial analysis of protein and RNA
-Analysis of tissue microarrays (TMAs)
Imaging Equipment:
• Instruments
-Leica Aperio AT2
-Akoya Vectra 3 Multispectral Imaging System
-Akoya PhenoImager HT 2.0 Multispectral Imaging System
-Evident/Olympus VS 200 Whole Slide Imaging System
-ThermoFisher Evos FL
• Software
-Indica Labs Halo/Halo Link
-Definiens Tissue Studio/Developer
-Akoya inForm/Phenochart
-Qupath
Laser Capture Microdissection Services:
• Training on use of Leica LMD7000 LCM instrument
• Training on identification of regions of interest on tissue slides using H&E or toluidine blue stained slides or images
Tissue Microarray Services:
• Design consulation
• TMA construction
Samples are accepted anytime in MSB-E316. Please leave your samples on the cart. You must have an order in iLabs to leave your sample. Please label the sample box with the project number and requester.
Turnaround time for brightfield scanning is usually 2 working days. If you have a time-sensitive project, please inquire about the turnabout time when placing an order. Advanced image analysis projects need to be discussed at the time of submission.
Peter Gann, MD, ScD
Academic Director
(312) 355-3723
pgann@uic.edu
| Hours | Location |
|
Monday-Thursday 9am-5pm |
College of Medicine Research Building
|
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Ryan Deaton |
312-355-4338
|
rdeaton@uic.edu
|
COMRB 6094
|
