Welcome!
Spatial & Genome Technologies Core (SGTC) is run by Melissa Leone, Director of Instrumentation & Infrastructure, Spatial & Genome Technologies Core.
What do we do?
Spatial & Genome Technologies Core provides expertise and advises investigators on developing projects on single cell transcriptomics at a reasonable price.
We engage in a formal collaboration with researchers to make sure the quality of their sample meets the standard requirement and that the application used is the best suited approach to address their questions.
We work closely with 10x support team to ensure the success of the project.
We meet with researchers before beginning their projects to ensure the most accurate and efficient results. We follow-up on their findings and provide feedback on different steps of the project.
Our single cell services include single cell RNAseq using microfluidic devices via the Chromium Single Cell System from 10x Genomics. Specifically, the Chromium Single Cell 3’ Solution provides a comprehensive gene expression profiling. For single cell scATAC-Seq, CITE-seq, Flex and Spatial Transcriptomics please inquire
We offer full bioinformatics support centered on single cell data analysis.
10x Genomics Controller Drop-seq 10x Genomics Chromium X
The Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. Powered by Next GEM technology, the Chromium Controller enables integrated analysis of single cells at massive scale.
Chromium X is a flexible instrument, with the most advanced hardware and wireless connectivity. It runs all single cell assays, including new high-throughput assays.
| Hours | Location |
|
Monday - Friday |
Room E616, MSB |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| SCS |
Staff Email
|
SCS@uic.edu
|
Room E616 MSB, 935 S Wolcott Ave., Chicago, IL, 60612
|
| Service Prices |
| ► Consultation (1) | |||
| Name | Description | Price | |
|---|---|---|---|
| Consultation |
meet to discuss project and their goals ~30 min |
Internal
$0.00
each
External (Academic US ONLY) $0.00 each External (Corporate and Foreign) $0.00 each External CBC $0.00 each External Collaboration (A) $0.00 each |
|
| ► Optimization (3) | |||
| Name | Description | Price | |
| Mock run CITE-seq Request - 2 samples |
Assessment of sample quality prior to scheduling the CITEseq experiment Price per run |
Inquire | |
| Test Count - Cell viability assessment by AOPI staining (Fx7 automated cell counter) |
Assessment of sample quality prior to scheduling the exp Price per sample |
Internal
$30.00
Sample
External (Academic US ONLY) $47.97 Sample External (Corporate and Foreign) $47.97 Sample |
|
| Mock run CITE-seq Request |
Assessment of sample quality prior to scheduling the CITEseq experiment Price per run |
Internal
$170.25
each
External (Academic US ONLY) $272.22 each External (Corporate and Foreign) $272.22 each |
|
| ► scRNAseq (13) | |||
| Name | Description | Price | |
| 10x Genomics 3’ Single Cell Gene Expression - 1 Sample |
microfluidic cell processing + cDNA amplification and QC + library preparation Price per sample |
Internal
$2,503.00
Sample
External (Academic US ONLY) $4,002.00 Sample External (Corporate and Foreign) $4,002.00 Sample |
|
| 10x Genomics 3’ Single Cell Gene Expression - 2 Samples |
microfluidic cell processing + cDNA amplification and QC + library preparation |
Internal
$4,570.00
each
External (Academic US ONLY) $7,308.00 each External (Corporate and Foreign) $7,308.00 each |
|
| 10x Genomics 3’ Single Cell Gene Expression - 3 Samples |
microfluidic cell processing + cDNA amplification and QC + library preparation |
Internal
$6,637.00
each
External (Academic US ONLY) $10,613.00 each |
|
| 10x Genomics 3’ Single Cell Gene Expression - 4 Samples |
microfluidic cell processing + cDNA amplification and QC + library preparation |
Internal
$8,704.00
each
External (Academic US ONLY) $13,918.00 each External (Corporate and Foreign) $13,918.00 each |
|
| 10x Genomics 3’ Single Cell Gene Expression - 4 Samples (HT kit) |
microfluidic cell processing + cDNA amplification and QC + library preparation |
Inquire | |
| 10x Genomics 3’ Single Cell Gene Expression - 6 Samples (HT kit) |
microfluidic cell processing + cDNA amplification and QC + library preparation |
Inquire | |
| 10x Genomics 3’ Single Cell Gene Expression - 8 Samples (HT kit) |
microfluidic cell processing + cDNA amplification and QC + library preparation |
Inquire | |
| 10x Genomics Single Cell ATAC v2 - 3 samples |
nuclei isolation + microfluidic cell processing + library preparation |
Inquire | |
| 10x Genomics Single Cell ATAC v2 - 6 samples |
nuclei isolation + microfluidic cell processing + library preparation |
Inquire | |
| 10x Genomics Single Cell ATAC v2 - 7 samples |
nuclei isolation + microfluidic cell processing + library preparation |
Inquire | |
| 10x Genomics Single Cell Fixed RNA profile- 4 Samples (Singleplex) |
QC + hybridization + microfluidic cell processing + library preparation + websummary |
Inquire | |
| 10x Genomics 3’ Single Cell Gene Expression + Cell Surface Protein Labeling- 2 Samples |
CITE-Seq: Cell Staining + microfluidic cell processing + cDNA amplification and QC + library preparation (gene expression and Cell Surface Protein) |
Inquire | |
| 10x Genomics 3’ Single Cell Gene Expression + Cell Surface Protein Labeling- 3 Samples |
CITE-Seq: Cell Staining + microfluidic cell processing + cDNA amplification and QC + library preparation (gene expression and Cell Surface Protein) |
Inquire | |
| ► Sequencing (6) | |||
| Name | Description | Price | |
| Library QC for 1 pooled library |
clean-up, Qubit, tapestation and qPCR on pooled library |
Internal
$85.00
each
External (Academic US ONLY) $136.00 each External (Corporate and Foreign) $136.00 each |
|
| Library QC for 2-8 samples |
QC on individual libraries, pooling + clean-up, Qubit, tapestation and qPCR on pooled library |
Internal
$221.62
each
External (Academic US ONLY) $354.37 each External (Corporate and Foreign) $354.37 each |
|
| Library QC for 2-8 samples (qPCR on individual libraries) |
library QC+ qPCR quantification, pooling + clean-up, Qubit, tapestation and qPCR on pooled library |
Inquire | |
| Nova X Plus 10B , Paired-reads, 28x90 |
1.2B clusters per lane |
Internal
$1,690.00
each
External (Academic US ONLY) $2,702.00 each External (Corporate and Foreign) $2,702.00 each External CBC $1,690.00 each External Collaboration (A) $1,859.00 each |
|
| Nova X Plus 10B, Paired-reads, 2x150 |
1.2B clusters per lane
|
Internal
$2,190.00
each
External (Academic US ONLY) $3,502.00 each External (Corporate and Foreign) $3,502.00 each External CBC $2,190.00 each External Collaboration (A) $2,409.00 each |
|
| Nova X Plus 25B, 2x150 |
3B clusters per lane |
Internal
$3,290.00
each
External (Academic US ONLY) $5,261.00 each External (Corporate and Foreign) $5,261.00 each External CBC $3,290.00 each External Collaboration (A) $3,619.00 each |
|
| ► Data Analysis (2) | |||
| Name | Description | Price | |
| Genome alignment and generation of count matrix |
Alignment, filtering & counting Price per sample |
Internal
$50.00
Sample
External (Academic US ONLY) $80.00 Sample External (Corporate and Foreign) $80.00 Sample |
|
| Genome alignment and generation of count matrix - CITEseq |
Alignment, filtering & counting (gene expression + cell surface protein expression) Price per sample |
Inquire | |
| ► Advance Bioinformatics Analysis (8) | |||
| Name | Description | Price | |
| Data management and storage | Inquire | ||
| Data management, storage and back up |
price for 6 months |
Inquire | |
| Project execution |
Price per project |
Internal
$618.00
each
External (Academic US ONLY) $988.00 each External (Corporate and Foreign) $988.00 each External CBC $618.00 each External Collaboration (A) $679.80 each |
|
| Data QC |
price per sample |
Inquire | |
| Speudo-time/trajectories (Monocle/Slingshot) |
less than 40,000 cells |
Inquire | |
| Cell-Cell communication NicheNet |
price for project execution |
Inquire | |
| Cell-cell communication (CellChat) |
price for project execution |
Inquire | |
| Additional bioinformatics support |
Price per hour |
Internal
$85.00
/hr
External (Academic US ONLY) $135.92 /hr External (Corporate and Foreign) $135.92 /hr |
|
